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翌圣生物科技（上海）股份有限公司
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- 曹女士
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- 400-6111-883
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- 售后：
- 4006-111-883
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- 86-21-34615995
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產品簡介
| 供貨周期 | 現貨 | 貨號 | 10661ES |
|---|---|---|---|
| 應用領域 | 醫(yī)療衛(wèi)生,生物產業(yè) |
產品介紹
Product description
This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and provided in a liquid form.
Specifications
Expression Host | Recombinant E. coli with Bas I gene |
Reaction Temperature | 37℃ |
Storage Buffer | 10mM Tris-HCl, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.2mg/ml OsrHSA pH 7.4±0.2 (25℃) |
Unit Definition | 1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system. |
Application | 1.Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G); 2.Digestion of DNA to obtain specific sticky ends； 3.Linearize plasmid template before in-vitro transcription. |
Components
Components No.
| Name | 10661ES03 (1 KU) | 10661ES10 (10 KU) | 10661ES60 (100 KU) |
10661 | Bsa I GMP-grade (20 U/μL) | 50 μL | 500 μL | 5 mL |
Storage
This product should be stored at -25 ~ -15℃ for two years.
Instructions
Experimental methods
50 μL reaction system
This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.
1. Add the following components in sequence:
Components | Volume |
Plasmid DNA | 1-2 μg |
10×Digestion Buffer 4 | 5.0 μL |
Bsa I (20 U/μL) | 1.0 μL |
RNase-free ddH2O | Up to 50 μL |
【Note】10× Digestion Buffer 4(Cat#10668ES): 500 mM Potassium Acetate，200 mM Tris-acetate，100 mM Magnesium Acetate，1 mg/ml OsrHSA, pH7.9@25℃
2. Incubate at 37°C 1 h;
3. DNA linearization is complete, and subsequent experiments can be performed.
Notes
1. Heat inactivation condition: incubate at 80°C for 20min.
2. Please operate with lab coats and disposable gloves，for your safety.
